Search results for "16S sequencing"

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Recurrent inoculation: a strategy for a better survival of Pseudomonas fluorescens strain in soil

2022

A growing interest is shown for microbial inoculants as a promising alternative to agrochemicals for sustainable agricultural. However, biotic and abiotic stresses are known to affect their establishment and effectiveness, leading to inconsistent performance in field conditions. At the same time, a poor survival of the strain is often observed, which constitutes a main hamper for bacterial inoculant development. We hypothesize that recurrent inoculations would improve the survival of the strain in soil. For this purpose, we tested different scenarios of inoculation of the plant growth promoting rhizobacteria Pseudomonas fluorescens in soil microcosms: a single inoculation, two inoculations …

16S sequencing[SDV] Life Sciences [q-bio]Microbial inoculantqPCR
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Gut Microbiota Analysis in Postoperative Lynch Syndrome Patients

2019

Lynch syndrome (LS) is a dominantly inherited condition with incomplete penetrance, characterized by high predisposition to colorectal cancer (CRC), endometrial and ovarian cancers, as well as to other tumors. LS is associated with constitutive DNA mismatch repair (MMR) gene defects, and carriers of the same pathogenic variants can show great phenotypic heterogeneity in terms of cancer spectrum. In the last years, human gut microbiota got a foothold among risk factors responsible for the onset and evolution of sporadic CRC, but its possible involvement in the modulation of LS patients’ phenotype still needs to be investigated. In this pilot study, we performed 16S rRNA gene sequencing of ba…

Microbiology (medical)medicine.medical_specialtyfecal biomarkersColorectal cancerlcsh:QR1-502Faecalibacterium prausnitziiGut floraMicrobiologyGastroenterologylcsh:Microbiology16S sequencing03 medical and health sciencesInternal medicinemedicineOriginal Research030304 developmental biology0303 health sciencesfecal microbiotabiology030306 microbiologyGenetic heterogeneityfood and beveragesCancermedicine.diseasebiology.organism_classificationPenetrancedigestive system diseasesLynch syndromeLynch syndromeBacteroides fragilishereditary cancer predispositionFrontiers in Microbiology
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